Ocean is the largest carbon pool on Earth and has huge potential for the carbon sink.The biologically-mediated transfer of particulate organic carbon (POC) to the deep sea, aka oceanic biological carbon pump, is a key pathway for oceanic carbon sequestration.The heterotrophic processes in the twilight zone (typically defined as the depth between the bottom of the euphotic zone and 1 000 m) consume more than 70% of the POC exported from the euphotic zone, and determine the carbon sequestration efficiency of the biological carbon pump.Therefore, quantification of the remineralization rate in the twilight zone is essential for the ocean budget assessment.Aiming at the carbon storage problem of marine biological pump, this paper focuses on the influence mechanism of heterotrophic processes in the twilight zone on marine carbon storage, reviews the global studies for remineralization rate estimation in the twilight zone, comprehensively analyzes the attenuation and remineralization of POC in the twilight zone, and looks forward to the application of relevant novel technologies.